Human HE4/WFDC2 ELISA Kit

Sensitivity

2.8 pg/mL

Range

46.88-3000 pg/mL

Reactivity

Human

Cat No : KE00265

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Synonyms

WFDC2, HE4, dJ461P17.6, WAP5



Product Information

KE00265 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HE4/WFDC2 ELISA kit is to be used to detect and quantify protein levels of endogenous human HE4/WFDC2. An antibody specific for HE4/WFDC2 has been pre-coated onto the microwells. The HE4/WFDC2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HE4/WFDC2 is added to detect the captured HE4/WFDC2 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human HE4/WFDC2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Saliva,Urine
Assay type Sandwich
Sensitivity 2.8 pg/mL
Range 46.88-3000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 10406

Recovery

Sample TypeAverageRange
Human plasma99%80%-120%
Urine99%80%-128%
Saliva99%77%-106%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1202,903.891.53.1
220725.521.02.9
320177.14.52.5

InterAssay

Samplenmean ( pg/mL)SDCV%
1242,776.2150.15.4
224685.322.53.3
324172.616.89.7

Background Information

Human epididymis protein 4 (HE4), also known as WFDC2, was originally identified as a transcript exclusively expressed in the epididymis and was proposed as a specific marker for this tissue. HE4 is a member of the Whey Acidic Protein (WAP) domain family of proteins. HE4 is expressed in a number of normal human tissues outside of the male reproductive system, including the trachea, lung and nasal epithelium and is also found in a subset of pulmonary epithelial-derived tumour cell lines. At present, HE4 serves as a serum biomarker of ovarian cancer, and has better sensitivity and specificity than CA125.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms WFDC2, HE4, dJ461P17.6, WAP5