Human HSP70 ELISA Kit
Cat no : KE00059
Synonyms
Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B
Validation Data Gallery
Product Information
KE000059 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP70 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP70. The assay recognizes human HSP70. An antibody specific for HSP70 has been pre-coated onto the microwells. The HSP70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HSP70 is added to detect the captured HSP70 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HSP70 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3303 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 86% | 76%-98% |
| Human plasma | 104% | 92%-124% |
| Cell culture supernatants | 97% | 80%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 182.3 | 7.7 | 4.2 |
| 2 | 20 | 448.4 | 18.2 | 4.1 |
| 3 | 20 | 1,627.2 | 102.4 | 6.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 181.9 | 11.1 | 6.1 |
| 2 | 24 | 465.4 | 23.4 | 5.0 |
| 3 | 24 | 1,639.4 | 90.5 | 5.5 |
Background Information
HSP70 is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, refolding, transport, and targeting. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Increased serum HSP70 levels are also associated with progression of multiple diseases, including atherosclerosis, heart failure, and diabetes.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Clin Invest Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. | ||
Transl Lung Cancer Res Increased plasma levels of damage-associated molecular patterns during systemic anticancer therapy in patients with advanced lung cancer. | ||
Cancer Med Changes in serum DAMPs and cytokines/chemokines during near-infrared photoimmunotherapy for patients with head and neck cancer | ||
Eur J Appl Physiol New Zealand blackcurrant extract modulates the heat shock response in men during exercise in hot ambient conditions | ||
Commun Biol Metabolic surgery improves insulin resistance through the reduction of gut-secreted heat shock proteins. |
