Human HSP90 ELISA Kit
Cat No : KE00054
Synonyms
Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2
Validation Data Gallery
Product Information
KE00054 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP90 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP90. The assay recognizes human HSP90. An antibody specific for HSP90 has been pre-coated onto the microwells. The HSP90 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HSP90 is added to detect the captured HSP90 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human HSP90 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates, Urine |
| Assay type | Sandwich |
| Sensitivity | 90 pg/mL |
| Range | 125-8000 pg/mL, 250-16000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3320 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 105% | 81%-124% |
| Human plasma | 100% | 82%-119% |
| Cell culture supernatants | 108% | 92%-126% |
| Cell lysates | 97% | 80%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5,941.0 | 463.1 | 7.8 |
| 2 | 20 | 1,655.7 | 77.2 | 4.7 |
| 3 | 20 | 436.0 | 30.5 | 7.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 7,834.6 | 752.1 | 9.6 |
| 2 | 24 | 2,133.4 | 163.6 | 7.7 |
| 3 | 24 | 525.3 | 31.2 | 5.9 |
Background Information
HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int Immunopharmacol Up-regulation of HSP90α in HDM-induced asthma causes pyroptosis of airway epithelial cells by activating the cGAS-STING-ER stress pathway | ||
Sci Rep HSP90 promotes tumor associated macrophage differentiation during triple-negative breast cancer progression |