Human IDO1 ELISA Kit
Validation Data Gallery
Product Information
KE00229 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IDO1 ELISA kit is to be used to detect and quantify protein levels of endogenous IDO1. The assay recognizes human IDO1. An antibody specific for IDO1 has been pre-coated onto the microwells. The IDO1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human IDO1 is added to detect the captured human IDO1 protein. For signal development, HRP-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IDO1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell cuture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.03 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3620 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell cuture supernatants | 103% | 87%-119% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.02 | 0.49 | 5.44 |
| 2 | 20 | 2.25 | 0.08 | 3.76 |
| 3 | 20 | 0.52 | 0.04 | 8.36 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 8.90 | 0.72 | 8.06 |
| 2 | 24 | 2.37 | 0.24 | 10.09 |
| 3 | 24 | 0.58 | 0.06 | 9.73 |
Background Information
Indoleamine 2, 3-dioxygenase 1 (IDO1) is a rate-limiting metabolic enzyme that converts the essential amino acid tryptophan (Trp) into downstream catabolites known as kynurenines. IDO1 can decrease the tryptophan and produce a series of toxic kynurenine metabolites to promote the immune toleration via GCN2 pathway, mTOR pathway, toxic effect of kynurenine and favoring differentiation of Tregs. The depletion of Trp, and the accumulation of Kyn have been proposed as mechanisms that contribute to the suppression of the immune response-primarily evidenced by in vitro study. IDO1 is therefore considered to be an immunosuppressive modulator and quantification of IDO1 metabolism may be critical to understanding its role in select immunopathologies, including autoimmune- and oncological-conditions, as well as for determining the potency of IDO1 enzyme inhibitors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Drug Resist Updat PDPN positive CAFs contribute to HER2 positive breast cancer resistance to trastuzumab by inhibiting antibody-dependent NK cell-mediated cytotoxicity | ||
Life Sci Synergetic impact of combined navoximod with cisplatin mitigates chemo-immune resistance via blockading IDO1+ CAFs-secreted Kyn/AhR/IL-6 and pol ζ-prevented CIN in human oral squamous cell carcinoma |