AuthentiKine™ Human IL-17F ELISA Kit
Sensitivity
14.4 pg/mL
Range
31.25-1000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00225 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-17F ELISA kit is to be used to detect and quantify protein levels of endogenous IL-17F. The assay recognizes human IL-17F. An antibody specific for IL-17F has been pre-coated onto the microwells. The IL-17F protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for IL-17F is added to detect the captured IL-17F protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | AuthentiKine™ Human IL-17F ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 14.4 pg/mL |
| Range | 31.25-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 112744 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 72%-89% | 80% |
| Cell culture supernatants | 79%-106% | 93% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 465.3 | 20.8 | 4.5 |
| 2 | 20 | 109.6 | 5.3 | 4.8 |
| 3 | 20 | 41.6 | 2.4 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 453.3 | 34.4 | 7.6 |
| 2 | 24 | 103.5 | 7.4 | 7.1 |
| 3 | 24 | 38.0 | 2.8 | 7.4 |
Background Information
The interleukin 17 (IL-17) family of cytokines contains 6 structurally related cytokines, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E and IL-17F. IL-17 family plays crucial roles in host defense against microbial organisms and in the development of inflammatory diseases. IL-17A is a pro-inflammatory cytokine that also has the capacity to promote angiogenesis and osteoclastogenesis. IL-17F shares the highest homology with IL-17A and signals via a receptor composed by the IL-17RA and IL-17RC subunits. IL-17A and IL-17F can form IL-17A/A or IL-17F/F homodimers, IL-17A/F heterodimers are also formed. IL-17A and IL-17F, produced by the Th17 CD4(+) T cell lineage, have been linked to a variety of inflammatory and autoimmune conditions. IL-17F levels are elevated in sera and lesional psoriatic skin compared to non-lesional tissue. IL-17F also has been implicated in the development of neutrophilic airway inflammation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cytokine ML 1, IL 17F, IL17F, IL-17F, interleukin 17F, ML 1, ML1 |