Human IL-12 p70 ELISA Kit
Cat No : KE00019
Synonyms
CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL-12, IL12 p70, IL-12 p70, IL12A, IL12p70, IL12-p70, Interleukin 12 subunit alpha, NFSK, NKSF1, P35
Validation Data Gallery
Product Information
KE00019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-12 p70. The assay recognizes human IL-12 p70. An antibody specific for IL-12 p70 has been pre-coated onto the microwells. The IL-12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-12 p70 is added to detect the captured IL-12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-12 p70 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine |
| Assay type | Sandwich |
| Sensitivity | 1.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3592 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 81% | 69%-89% |
| Human plamsa | 105% | 91%-124% |
| Cell culture supernatants | 102% | 96%-112% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 74.6 | 1.7 | 2.3 |
| 2 | 20 | 301.1 | 8.0 | 2.7 |
| 3 | 20 | 1262.9 | 102.0 | 8.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 98.4 | 8.7 | 8.8 |
| 2 | 24 | 394.6 | 37.0 | 9.4 |
| 3 | 24 | 1441.0 | 139.8 | 9.7 |
Background Information
Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40). IL-12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL-12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL-12, IL12 p70, IL-12 p70, IL12A, IL12p70, IL12-p70, Interleukin 12 subunit alpha, NFSK, NKSF1, P35 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Med Virol Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy | ||
Proc Natl Acad Sci U S A miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression. | ||
Cancer Lett MiR-26b-5p in small extracellular vesicles derived from dying tumor cells after irradiation enhances the metastasis promoting microenvironment in esophageal squamous cell carcinoma. | ||
Oncogenesis Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer. | ||
Life Sci Oncolytic virus Ad-TD-nsIL-12 inhibits glioma growth and reprograms the tumor immune microenvironment |