Human IL-13 ELISA Kit
Validation Data Gallery
Product Information
KE00020 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-13 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-13. The assay recognizes human IL-13. An antibody specific for IL-13 has been pre-coated onto the microwells. The IL-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-13 is added to detect the captured IL-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 1.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3596 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Plasma | 88% | 81%-95% |
| Cell culture supernatants | 84% | 73%-96% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 385.0 | 23.4 | 6.1 |
| 2 | 20 | 848.6 | 51.8 | 6.1 |
| 3 | 20 | 3,209.4 | 127.8 | 4.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 374.7 | 23.2 | 6.2 |
| 2 | 24 | 840.6 | 68 | 8.1 |
| 3 | 24 | 3,204.9 | 315.4 | 9.8 |
Background Information
Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | ALRH, BHR1, IL 13, IL13, IL-13, interleukin 13, NC30, P600 |
Publications
| Species | Sample Type | Title |
|---|---|---|
Oncogenesis Erlotinib overcomes paclitaxel-resistant cancer stem cells by blocking the EGFR-CREB/GRβ-IL-6 axis in MUC1-positive cervical cancer. | ||
J Asthma Allergy The Multiomics Analyses of Gut Microbiota, Urine Metabolome and Plasma Proteome Revealed Significant Changes in Allergy Featured with Indole Derivatives of Tryptophan. | ||
Sci Rep Paeoniflorin drives the immunomodulatory effects of mesenchymal stem cells by regulating Th1/Th2 cytokines in oral lichen planus | ||
J Invest Dermatol UBE2L3 Reduces TRIM21 Expression and Interleukin-1β Secretion in Epidermal Keratinocytes and Improves Psoriasis-like Skin |