Human IL-4 ELISA Kit
Cat No : KE00016
Synonyms
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra
Validation Data Gallery
Product Information
KE00016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-4 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-4. The assay recognizes human IL-4. An antibody specific for IL-4 has been pre-coated onto the microwells. The IL-4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-4 is added to detect the captured IL-4 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 2.2 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3565 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 103% | 86%-116% |
| Cell culture supernatants | 95% | 83%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 777.0 | 45.1 | 5.8 |
| 2 | 20 | 225.5 | 6.4 | 2.8 |
| 3 | 20 | 43.3 | 2.4 | 5.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 703.8 | 33.8 | 4.8 |
| 2 | 24 | 212.2 | 13.3 | 6.2 |
| 3 | 24 | 42.4 | 1.9 | 4.5 |
Background Information
Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL-4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL-4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra |
Publications
| Species | Sample Type | Title |
|---|---|---|
Front Cell Infect Microbiol The effect of gut microbiota dysbiosis on patients with preeclampsia | ||
J Asthma Allergy The Multiomics Analyses of Gut Microbiota, Urine Metabolome and Plasma Proteome Revealed Significant Changes in Allergy Featured with Indole Derivatives of Tryptophan. | ||
Oncol Lett Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia. | ||
Exp Ther Med Changes in expression of p53 and inflammatory factors in patients with ulcerative colitis. | ||
Sci Rep Paeoniflorin drives the immunomodulatory effects of mesenchymal stem cells by regulating Th1/Th2 cytokines in oral lichen planus | ||
Radiat Environ Biophys Immune status of people living in the Tande-Tande sub-village (Indonesia), an area with high indoor radon concentration |