Human IL-6R ELISA Kit
Cat No : KE00118
Synonyms
CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80
Validation Data Gallery
Product Information
KE00118 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL6R ELISA kit is to be used to detect and quantify protein levels of endogenous IL6R. The assay recognizes human IL6R. An antibody specific for IL6R has been pre-coated onto the microwells. The IL6R protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL6R is added to detect the captured IL6R protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
Product name | Human IL-6R ELISA Kit |
Tests | 1 X 96 well plate |
Sample type | Serum, Plasma, Cell culture supernatants, Urine |
Assay type | Sandwich |
Sensitivity | 0.1 pg/mL |
Range | 7.8 - 500 pg/mL |
Reactivity | Human |
Tested applications | Sandwich ELISA |
Gene ID (NCBI) | 3570 |
Recovery
Sample Type | Average | Range |
---|---|---|
Human serum | 89% | 80%-119% |
Cell culture supernatants | 94% | 82%-108% |
Urine | 97% | 80%-108% |
IntraAssay
Sample | n | mean ( pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 20 | 236.9 | 8.2 | 3.5 |
2 | 20 | 61.6 | 1.8 | 2.9 |
3 | 20 | 15.1 | 0.5 | 3.2 |
InterAssay
Sample | n | mean ( pg/mL) | SD | CV% |
---|---|---|---|---|
1 | 24 | 271.4 | 12.9 | 4.8 |
2 | 24 | 67.2 | 3.2 | 4.8 |
3 | 24 | 16.0 | 0.8 | 5.2 |
Background Information
Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge. IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor. Expression of gp130 is found in almost all organs while expression of IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells). Soluble form of IL-6R has been found in human serum and urine.
Properties
Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
Synonyms | CD126, gp80, IL 6 receptor subunit alpha, IL 6R 1, IL 6R alpha, IL 6R subunit alpha, IL 6RA, IL6R, IL-6R, IL6RA, interleukin 6 receptor, Membrane glycoprotein 80 |
Publications
Species | Sample Type | Title |
---|---|---|
Cancer Lett Induction of IL-6Rα by ATF3 enhances IL-6 mediated sorafenib and regorafenib resistance in hepatocellular carcinoma. | ||
Mol Med IL-33/ST2 axis of human amnion fibroblasts participates in inflammatory reactions at parturition | ||
Front Immunol E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism |