Human JNK ELISA Kit
Sensitivity
0.1 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00072
Synonyms
c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1
Validation Data Gallery
Product Information
KE00072 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The JNK ELISA kit is to be used to detect and quantify protein levels of endogenous JNK. The assay recognizes human JNK. An antibody specific for JNK has been pre-coated onto the microwells. The JNK protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for JNK is added to detect the captured JNK protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human JNK ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.1 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5599 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell lysates | 100% | 85%-110% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 82.0 | 5.5 | 6.7 |
| 2 | 20 | 28.5 | 0.8 | 2.8 |
| 3 | 20 | 6.3 | 0.3 | 5.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 86.0 | 6.3 | 7.3 |
| 2 | 24 | 28.0 | 2.0 | 7.2 |
| 3 | 24 | 5.5 | 0.5 | 8.9 |
Background Information
JNK is also named as MAPK8 (Mitogen-activated protein kinase 8), PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. JNK is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. JNK functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. JNK activity is abnormally elevated in obesity. Furthermore, an absence of JNK results in decreased adiposity, significantly improved insulin sensitivity, and enhanced insulin receptor signaling capacity in 2 different models of mouse obesity, including ob/ob. JNK is a crucial mediator of obesity and insulin resistance and a potential target for therapeutics. The ELISA kit is suitable for testing cell lysates.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
