Human LDH-B ELISA Kit
Sensitivity
0.04 ng/mL
Range
0.625-40 ng/mL
Reactivity
Human
Cat No : KE00287
Synonyms
lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5
Validation Data Gallery
Product Information
KE00287 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The LDH-B ELISA kit is to be used to detect and quantify protein levels of endogenous LDH-B. The assay recognizes human LDH-B. An antibody specific for LDH-B has been pre-coated onto the microwells. The LDH-B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for LDH-B is added to detect the captured LDH-B protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human LDH-B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell Lysate |
| Assay type | Sandwich |
| Sensitivity | 0.04 ng/mL |
| Range | 0.625-40 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3945 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 113% | 86%-130% |
| Cell Lysate | 103% | 92%-122% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 18.65 | 0.72 | 3.84 |
| 2 | 20 | 4.56 | 0.20 | 4.47 |
| 3 | 20 | 0.99 | 0.08 | 8.25 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 17.81 | 0.79 | 4.46 |
| 2 | 24 | 4.49 | 0.14 | 3.16 |
| 3 | 24 | 1.13 | 0.09 | 8.15 |
Background Information
LDH is a tetrameric enzyme that is comprised by four subunits of two types: muscle/anaerobic (LDH-A) and heart/aerobic (LDH-B). Various combinations of LDH-A subunits and LDH-B subunits (which are produced from a different gene) can form homo- or heterotetramers, and assemble into five different combinations LDH-1 (4B), LDH-2 (3B,1A), LDH-3 (2B,2A), LDH-4 (1B,3A), and LDH-5 (4A). LDH-A converts pyruvate to lactate and is expressed in the skeletal muscle, while LDH-B converts lactate to pyruvate and is expressed more in the heart muscle.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | lactate dehydrogenase B, LDH B, LDH H, LDH heart subunit, LDHB, TRG 5 |