Human Leptin ELISA Kit
Validation Data Gallery
Product Information
KE00095 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human leptin ELISA kit is to be used to detect and quantify protein levels of endogenous human . The assay recognizes human leptin. An antibody specific for human leptin has been pre-coated onto the microwells. The human leptin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human leptin is added to detect the captured human leptin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Leptin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Cell lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.0 pg/mL |
| Range | 15.6 - 500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3952 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 94% | 82%-113% |
| Cell culture supernatants | 98% | 80%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 79.7 | 2.8 | 3.5 |
| 2 | 20 | 182.7 | 5.8 | 3.2 |
| 3 | 20 | 461.7 | 20.9 | 4.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 84.3 | 1.9 | 2.2 |
| 2 | 24 | 185.1 | 4.6 | 2.5 |
| 3 | 24 | 461.7 | 15.4 | 3.3 |
Background Information
Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance. Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LEP, OB, Obese protein, Obesity factor, OBS |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Exp Eye Res High-throughput transcriptional profiling combined with angiogenesis antibody array analysis in an orbital venous malformation cohort. | ||
Antioxidants (Basel) Transwell Culture with Adipose Tissue-Derived Stem Cells and Fertilized Eggs Mimics the In Vivo Development of Fertilized Eggs to Blastocysts in the Fallopian Tube: An Animal Study | ||
Clin Pediatr Endocrinol Platelet and white blood cell counts correlate with leptin and body mass index in Japanese adolescents |
