Human MMP-13 ELISA Kit
Cat no : KE00078
Synonyms
CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13, MMP-13
Validation Data Gallery
Product Information
KE00078 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-13 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-13. The assay recognizes human MMP-13. An antibody specific for MMP-13 has been pre-coated onto the microwells. The MMP-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MMP-13 is added to detect the captured MMP-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MMP-13 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.2 pg/mL |
| Range | 93.8-6000 pg/ml |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4322 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 90% | 82%-100% |
| Cell culture supernatants | 86% | 79%-92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 928.6 | 69.5 | 7.5 |
| 2 | 20 | 1,630.0 | 101.7 | 6.2 |
| 3 | 20 | 3,808.3 | 227.1 | 6.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,002.6 | 78.1 | 7.8 |
| 2 | 24 | 1,833.0 | 111.3 | 6.1 |
| 3 | 24 | 4,422.9 | 363.0 | 8.2 |
Background Information
MMP-13 (Matrix metalloproteinase 13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP13 is considered to play a role in the tumor cell proliferation and invasion. MMP13 is a potential prognostic marker for colorectal cancer, breast cancer and other diseases (PMID: 20878116, PMID: 19787229).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13, MMP-13 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cancer Lett PLD1 promotes tumor invasion by regulation of MMP-13 expression via NF-κB signaling in bladder cancer. | ||
J Clin Med Zinc Administration and Improved Serum Markers of Hepatic Fibrosis in Patients with Autoimmune Hepatitis. | ||
Int J Mol Sci Exosomes Derived from Hypoxia-Cultured Human Adipose Stem Cells Alleviate Articular Chondrocyte Inflammaging and Post-Traumatic Osteoarthritis Progression | ||
Chin Med J (Engl) Development of a novel HER2-CAR monocyte cell therapy with controllable proliferation and enhanced anti-tumor efficacy | ||
Mol Carcinog Breast cancer malignancy is governed by regulation of the macroH2A2/TM4SF1 axis, the AKT/NF-κB pathway, and elevated MMP13 expression | ||
