Human Total MMP-7 ELISA Kit
Sensitivity
3.0 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Cat no : KE00219
Synonyms
Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MMP-7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase
Validation Data Gallery
Product Information
KE00219 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total MMP-7 ELISA kit is to be used to detect and quantify protein levels of endogenous Total MMP-7. The assay recognizes human Total MMP-7. An antibody specific for Total MMP-7 has been pre-coated onto the microwells. The Total MMP-7 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Total MMP-7 is added to detect the captured human Total MMP-7 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total MMP-7 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Saliva, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.0 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4316 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 91% | 75%-119% |
| Human saliva | 102% | 75%-125% |
| Human urine | 101% | 78%-129% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,731.7 | 107.1 | 6.2 |
| 2 | 20 | 380.7 | 12.9 | 3.4 |
| 3 | 20 | 100.5 | 6.9 | 6.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,921.6 | 206.0 | 10.7 |
| 2 | 24 | 432.9 | 36.0 | 8.5 |
| 3 | 24 | 104.8 | 9.8 | 9.3 |
Background Information
Matrix metalloproteinase-7 (MMP-7)/ matrilysin is a member of the MMP family, but is structurally different from the other MMPs by virtue of the absence of a conserved COOH-terminal protein domain. MMPs are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and cancer metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP-7 degrades proteoglycans, fibronectin, elastin and casein, and is involved in wound healing, tumor progression, pulmonary fibrosis, and development of choroidal neovascularization in age-related macular degeneration. The expression of MMP-7 is increased in most tumors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Matrilysin, Matrin, Matrix metalloproteinase 7, MMP 7, MMP7, MMP-7, MPSL1, PUMP 1, Pump 1 protease, PUMP1, Uterine metalloproteinase |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
