Human N-cadherin ELISA Kit
Sensitivity
5.6 pg/mL
Range
46.88-3000 pg/mL
Reactivity
Human
Cat no : KE00114
Synonyms
Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin
Validation Data Gallery
Product Information
KE00114 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The N-caderin ELISA kit is to be used to detect and quantify protein levels of endogenous N-caderin. The assay recognizes human N-caderin. An antibody specific for N-caderin has been pre-coated onto the microwells. The N-caderin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human N-caderin is added to detect the captured human N-caderin protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human N-cadherin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 5.6 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 1000 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 96% | 85%-107% |
| Human serum | 99% | 77%-117% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,413.1 | 48.5 | 3.4 |
| 2 | 20 | 395.9 | 11.8 | 3.3 |
| 3 | 20 | 91.9 | 3.9 | 4.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,347.9 | 72.2 | 5.4 |
| 2 | 24 | 348.7 | 13.6 | 3.9 |
| 3 | 24 | 89.0 | 3.1 | 3.5 |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes. N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity. Soluble N-cadherin (sN-CAD) is found in biological fluids, including serum, seminal fluid samples, and urine. It has been shown that significantly higher amounts of serum sN-CAD are present in cancer patients than in persons with no evidence of disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
