Human NGAL ELISA Kit
Cat no : KE00144
Synonyms
LCN2, lipocalin 2, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL
Validation Data Gallery
Product Information
KE00144 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous NGAL. The assay recognizes human NGAL. An antibody specific for NGAL has been pre-coated onto the microwells. The NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for NGAL is added to detect the captured NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatants, Urine, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 3.3 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3934 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 93% | 79%-106% |
| cell cuture supernatants | 88% | 73%-103% |
| urine | 92% | 84%-102% |
| saliva | 97% | 75%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,493.7 | 166.0 | 6.7 |
| 2 | 20 | 655.1 | 16.6 | 2.5 |
| 3 | 20 | 154.8 | 11.0 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,550.7 | 78.4 | 3.1 |
| 2 | 24 | 625.4 | 17.5 | 2.8 |
| 3 | 24 | 150.1 | 7.4 | 4.9 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. NGAL exists as a monomer, a homodimer and a disulfide-linked heterodimer in cells, covalently conjugated with gelatinase (matrix metalloproteinase-9, MMP-9) in order to decrease its degradation. Several roles of NGAL was identified, including iron trafficking, chemotactic, and promote cell differentiation and proliferation. NGAL was originally found in human neutrophils, and expressed in tissues containing renal, uterus, liver, spleen, as well as cardiomyocytes, neurons and immune cells. Studies showed that NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation and metabolic diseases, and it can be detected in human serum, saliva and urine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | LCN2, lipocalin 2, 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Crit Care A translational study of Galectin-3 as an early biomarker and potential therapeutic target for ischemic-reperfusion induced acute kidney injury. | ||
Sci Rep Preliminary evidence of immune infiltration and neutrophil degranulation in peripheral blood of non-obese OSA patients related to cognitive decline | ||
Front Neurol Lipocalin-2 in preoperative cerebrospinal fluid is a biomarker for postoperative delirium after hip fracture surgery in older adults: a prospective cohort study |
