Human Osteopontin/OPN ELISA Kit
Cat No : KE00233
Synonyms
BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin
Validation Data Gallery
Product Information
KE00233 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Osteopontin/OPN ELISA kit is to be used to detect and quantify protein levels of endogenous Osteopontin/OPN. The assay recognizes human Osteopontin/OPN. An antibody specific for Osteopontin/OPN has been pre-coated onto the microwells. The Osteopontin/OPN protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Osteopontin/OPN is added to detect the captured human Osteopontin/OPN protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Osteopontin/OPN ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Human milk |
| Assay type | Sandwich |
| Sensitivity | 1.85 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6696 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 83%-117% |
| Human plasma | 94% | 77%-107% |
| Cell culture supernatants | 96% | 84%-117% |
| Urine | 92% | 77%-112% |
| Human milk | 93% | 86%-100% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,000.9 | 30.3 | 3.0 |
| 2 | 20 | 232.2 | 10.4 | 4.5 |
| 3 | 20 | 54.5 | 2.3 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 942.4 | 38.1 | 4.0 |
| 2 | 24 | 214.7 | 11.8 | 5.5 |
| 3 | 24 | 47.6 | 3.4 | 7.1 |
Background Information
Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BNSP, Bone sialoprotein 1, BSPI, ETA 1, Nephropontin, OPN, Osteopontin, secreted phosphoprotein 1, SPP 1, SPP1, Urinary stone protein, Uropontin |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Mol Sci Proteomic Changes Induced by the Immunosuppressant Everolimus in Human Podocytes | ||
Front Immunol OPN promotes pro-inflammatory cytokine expression via ERK/JNK pathway and M1 macrophage polarization in Rosacea | ||
Biochem Biophys Res Commun Pirfenidone and nintedanib exert additive antifibrotic effects by the SPP1-AKT pathway in macrophages and fibroblasts |