Human PCSK9/Proprotein Convertase 9 ELISA Kit

Sensitivity

0.01 ng/mL

Range

0.156-10 ng/mL

Reactivity

Human

Cat No : KE00181

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Synonyms

FH3, HCHOLA3, LDLCQ1, NARC 1, NARC1, PC9, PCSK9, Proprotein convertase 9



Product Information

KE00181 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PCSK9 ELISA kit is to be used to detect and quantify protein levels of endogenous PCSK9. The assay recognizes human PCSK9. An antibody specific for PCSK9 has been pre-coated onto the microwells. The PCSK9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PCSK9 is added to detect the captured PCSK9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human PCSK9/Proprotein Convertase 9 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Cell lysates
Assay type Sandwich
Sensitivity 0.01 ng/mL
Range 0.156-10 ng/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 255738

Recovery

Sample TypeAverageRange
Human serum93%82%-105%
Cell culture supernatants87%79%-104%
Cell lysates105%86%-115%

IntraAssay

Samplenmean ( ng/mL)SDCV%
1204.660.153.1
2201.260.021.9
3200.310.013.4

InterAssay

Samplenmean ( ng/mL)SDCV%
1244.910.234.6
2241.260.053.8
3240.270.026.7

Background Information

PCSK9, also named as NARC1 and PC9, belongs to the peptidase S8 family. PCSK9 is expressed and secreted by several tissues but principally by the liver, small intestines, and kidney(PMID 28587771). It may be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis. PCSK9 is a regulator of the LDL receptor and plasma cholesterol concentrations(PMID: 26293463).

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms FH3, HCHOLA3, LDLCQ1, NARC 1, NARC1, PC9, PCSK9, Proprotein convertase 9

Publications

SpeciesSample TypeTitle

Biomed Pharmacother

Novel ASO therapeutic target designed against hyperlipidemia via PCSK9 knockdown

Authors - Xinjian Yu