Human PDGF-AB ELISA Kit
Sensitivity
14.5 pg/mL
Range
31.25 - 1000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00222 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PDGF-AB ELISA kit is to be used to detect and quantify protein levels of endogenous PDGF-AB. The assay recognizes human PDGF-AB. An antibody specific for PDGF-AB has been pre-coated onto the microwells. The PDGF-AB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human PDGF-AB is added to detect the captured human PDGF-AB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PDGF-AB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 14.5 pg/mL |
| Range | 31.25 - 1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5154 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 101% | 78%-122% |
| Cell culture supernatants | 113% | 93%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 425.3 | 26.8 | 6.3 |
| 2 | 20 | 77.2 | 4.1 | 5.3 |
| 3 | 20 | 41.8 | 2.3 | 5.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 575.9 | 35.4 | 6.2 |
| 2 | 24 | 127.9 | 9.3 | 7.3 |
| 3 | 24 | 63.3 | 5.3 | 8.4 |
Background Information
Platelet-derived growth factor (PDGF) is one of the most abundant growth factors contained in the platelet alpha-granules and released upon platelet activation. It is also secreted by epithelial and mesenchymal cells. PDGF family consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. The PDGFR receptors are encoded by two genes: PDGFRA and PDGFRB. PDGFR is known to control growth, cell movement, angiogenesis and embryo development.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PDGF 1, PDGF A, PDGF AB, PDGF subunit A, PDGF1, PDGFA, PDGF-AB |