Human PDGF-BB ELISA Kit
Cat no : KE00161
Synonyms
Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, Proto oncogene c Sis, SIS, SSV
Validation Data Gallery
Product Information
KE00161 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PDGF-BB ELISA kit is to be used to detect and quantify protein levels of endogenous PDGF-BB. The assay recognizes human PDGF-BB. An antibody specific for PDGF-BB has been pre-coated onto the microwells. The PDGF-BB protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human PDGF-BB is added to detect the captured human PDGF-BB protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human PDGF-BB ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 3.9-250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5155 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 100% | 80%-126% |
| Cell culture supernatants | 111% | 70%-134% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 57.1 | 1.3 | 2.2 |
| 2 | 20 | 27.6 | 0.8 | 2.8 |
| 3 | 20 | 6.6 | 0.4 | 5.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 53.9 | 2.0 | 3.8 |
| 2 | 24 | 26.3 | 1.1 | 4.3 |
| 3 | 24 | 6.5 | 0.4 | 6.2 |
Background Information
Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Becaplermin, c sis, FLJ12858, PDGF 2, PDGF subunit B, PDGF2, PDGFB, Proto oncogene c Sis, SIS, SSV |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
QJM Predicting survival and immune microenvironment in colorectal cancer: a STAT signaling-related signature. | ||
Food Res Int Efficient expression of a cytokine combination in Saccharomyces cerevisiae for cultured meat production | ||
J Dent Calvaria defect regeneration via human periodontal ligament stem cells and prevascularized scaffolds in athymic rats | ||
Br J Cancer Pirfenidone alleviates fibrosis by acting on tumour-stroma interplay in pancreatic cancer | ||
J Nanobiotechnology ALPL regulates pro-angiogenic capacity of mesenchymal stem cells through ATP-P2X7 axis controlled exosomes secretion |
