Human/Mouse/Rat PIP5K1A ELISA Kit

Sensitivity

0.008 ng/mL

Range

0.156-10 ng/mL

Reactivity

Human/Mouse/Rat

Cat No : KE00227

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Synonyms

PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha



Product Information

KE00227 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PIP5K1A ELISA kit is to be used to detect and quantify protein levels of endogenous PIP5K1A. The assay recognizes human/mouse/rat PIP5K1A. An antibody specific for PIP5K1A has been pre-coated onto the microwells. The PIP5K1A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for PIP5K1A is added to detect the captured PIP5K1A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human/Mouse/Rat PIP5K1A ELISA Kit
Tests 1 X 96 well plate
Sample type Human serum, Human plasma, Mouse serum, Rat serum and Cell lysate
Assay type Sandwich
Sensitivity 0.008 ng/mL
Range 0.156-10 ng/mL
Reactivity Human/Mouse/Rat
Tested applications Sandwich ELISA
Gene ID (NCBI) 8394

Recovery

Sample TypeAverageRange
Human plasma87%80%-98%
Mouse serum102%76%-120%
Rat serum86%78%-97%
Cell lysate86%75%-99%

IntraAssay

Samplenmean ( ng/mL)SDCV%
1200.300.028.4
2201.170.032.6
3204.920.142.9

InterAssay

Samplenmean ( ng/mL)SDCV%
1240.280.026.5
2241.200.087.0
3245.570.386.8

Background Information

Phosphatidylinositol 4-phosphate 5-kinases (PIP5Ks) play diverse roles in the cellular biology of many organisms, including signal transduction, secretion and vesicular trafficking, and regulation of cytoskeleton assembly.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms PIP5K1 alpha, PIP5K1A, PIP5KIalpha, PtdIns(4)P 5 kinase 1 alpha