Human Phospho-P53 (Ser15) ELISA Kit
Sensitivity
2.3 ug/mL
Reactivity
Human
Cat no : KE40001
Synonyms
Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53
Validation Data Gallery
Product Information
KE40001 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Phospho-P53 (Ser15) ELISA kit is to be used to detect and quantify protein levels of endogenous Phospho-P53 (Ser15). The assay recognizes human Phospho-P53 (Ser15). An antibody specific for human P53 has been pre-coated onto the microwells. Phosphorylated sample is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for human Phospho-P53 (Ser15) is added to detect cell Lysate samples. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Phospho-P53 (Ser15) ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Cell lysates,Tissue Lysate,Tissue Homogenate 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.3 ug/mL |
| Range | N/A |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7157 |
Recovery
| Sample Type | Average | Range |
|---|
IntraAssay
| Sample | n | mean ( ug/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 150.0 | 4.9 | 3.3 |
| 2 | 20 | 20.2 | 1.5 | 7.4 |
InterAssay
| Sample | n | mean ( ug/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 149.7 | 15.9 | 10.6 |
| 2 | 24 | 19.9 | 2.1 | 10.6 |
Background Information
Stabilized p53 transcriptionally activates genes encoding proteins involved in cell cycle arrest, DNA repair, and/or apoptosis, and represses transcriptional activation of growth-promoting genes. Under physiological conditions, p53, in most cells, is expressed at a low or undetectable level, with a half-life of a few minutes. Under a condition of stress, such as DNA damage caused by ionizing radiation or cytotoxic agents, endogenous p53 is stabilized through a series of physiological responses, including ataxia telangiectasia mutated (ATM)/ATM and Rad3-related (ATR) activation, phosphorylation and acetylation of p53, and weakening of the binding of MDM2 to p53. Phosphorylation of serine 15 also prevents p53 from being exported from the nucleus and stimulates p53 transcriptional activity through the increased association with p300 coactivator.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
