Human Progranulin/PCDGF ELISA Kit
Sensitivity
0.05 ng/mL
Range
6.25-400 ng/mL
Reactivity
Human
Cat no : KE00260
Synonyms
GEP, GP88, granulin, Granulins, GRN, KE00260, PCDGF, PGRN, Proepithelin, Progranulin
Validation Data Gallery
Product Information
KE00260 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Progranulin/PCDGF ELISA kit is to be used to detect and quantify protein levels of endogenous Progranulin/PCDGF. The assay recognizes human Progranulin/PCDGF. An antibody specific for Progranulin/PCDGF has been pre-coated onto the microwells. The Progranulin/PCDGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for Progranulin/PCDGF is added to detect the captured Progranulin/PCDGF protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Progranulin/PCDGF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva, Urine, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.05 ng/mL |
| Range | 6.25-400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2896 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Saliva | 102% | 92%-123% |
| Human plamsa | 100% | 92%-110% |
| Cell culture supernatants | 115% | 100%-125% |
| Urine | 95% | 74%-124% |
| Human milk | 110% | 84%-127% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 57.29 | 1.61 | 2.8 |
| 2 | 20 | 102.27 | 4.25 | 4.2 |
| 3 | 20 | 189.93 | 7.59 | 4.0 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 56.16 | 2.84 | 5.1 |
| 2 | 24 | 96.77 | 5.2 | 5.4 |
| 3 | 24 | 182.62 | 13.12 | 7.2 |
Background Information
Progranulin, also known as Acrogranin, PCDGF, and Epithelin/Granulin Precursor, was first identified as a 593-aa secreted glycoprotein involved in the regulation of cancer progression and wound healing. It is a widely expressed glycoprotein that can undergo proteolysis to generate numerous Granulin peptides. Progranulin (PGRN) has been considered a growth factor-like molecule implicated in additional biological and pathological processes including early embryogenesis, inflammation, host defense, cartilage development and degradation.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | GEP, GP88, granulin, Granulins, GRN, KE00260, PCDGF, PGRN, Proepithelin, Progranulin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
