Human RBP4 ELISA Kit
Cat no : KE00056
Synonyms
Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4
Validation Data Gallery
Product Information
KE00056 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The RBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous RBP4. The assay recognizes human RBP4. An antibody specific for RBP4 has been pre-coated onto the microwells. The RBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for RBP4 is added to detect the captured RBP4 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RBP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 31.25-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 99% | 88%-114% |
| Urine | 104% | 93%-112% |
| Saliva | 111% | 94%-121% |
| Cell culture supernatants | 100% | 84%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 500.0 | 13.9 | 2.8 |
| 2 | 20 | 115.0 | 3.1 | 2.7 |
| 3 | 20 | 22.9 | 2.2 | 9.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 572.0 | 42.3 | 7.4 |
| 2 | 24 | 115.6 | 8.1 | 7.0 |
| 3 | 24 | 33.0 | 3.2 | 9.9 |
Background Information
RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some insulin-resistant states. Measurement of serum RBP4 could be used to assess the risk of insulin resistance, type 2 diabetes, obesity, and cardiovascular disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Exp Clin Cancer Res Gastric cancer-derived LBP promotes liver metastasis by driving intrahepatic fibrotic pre-metastatic niche formation | ||
Tuberculosis (Edinb) LDH and LV218 as biomarkers for diagnosing microbiologically positive tuberculous pleural effusions. |
