Human RBP4 ELISA Kit
Cat No : KE00056
Synonyms
Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4
Validation Data Gallery
Product Information
KE00056 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The RBP4 ELISA kit is to be used to detect and quantify protein levels of endogenous RBP4. The assay recognizes human RBP4. An antibody specific for RBP4 has been pre-coated onto the microwells. The RBP4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for RBP4 is added to detect the captured RBP4 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human RBP4 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 2.3 pg/mL |
| Range | 31.25-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5950 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 99% | 88%-114% |
| Urine | 104% | 93%-112% |
| Saliva | 111% | 94%-121% |
| Cell culture supernatants | 100% | 84%-118% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 500.0 | 13.9 | 2.8 |
| 2 | 20 | 115.0 | 3.1 | 2.7 |
| 3 | 20 | 22.9 | 2.2 | 9.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 572.0 | 42.3 | 7.4 |
| 2 | 24 | 115.6 | 8.1 | 7.0 |
| 3 | 24 | 33.0 | 3.2 | 9.9 |
Background Information
RBP4 (retinol-binding protein 4) is a carrier protein that transports vitamin A (retinol) from the liver to the peripheral tissues. Synthesized primarily by hepatocytes and adipocytes as a 21 kDa non-glycosylated protein, RBP4 is secreted into the circulation as a retinol-RBP4 complex. In plasma the RBP4-retinol complex is bound to transthyretin (TRR), which prevents prevent kidney filtration. Two truncated forms of RBP4, RBP4-L (truncated at Leu-183) and RBP4-LL (truncated at Leu-182 and Leu-183), exist by proteolytic process. RBP4-L and RBP4-LL, which do not bind TTR, are normally excreted into the urine but accumulate in the serum during renal failure. Urinary RBP4 has been reported as marker for glomerular disease. RBP4 also was identified as an adipokine that elevated in some insulin-resistant states. Measurement of serum RBP4 could be used to assess the risk of insulin resistance, type 2 diabetes, obesity, and cardiovascular disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Plasma retinol binding protein, PRBP, RBP, RBP4, Retinol binding protein 4 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Exp Clin Cancer Res Gastric cancer-derived LBP promotes liver metastasis by driving intrahepatic fibrotic pre-metastatic niche formation |