Human S100A8/S100A9 ELISA Kit
Cat no : KE00177
Synonyms
60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A
Validation Data Gallery
Product Information
KE00177 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The S100A8/S100A9 ELISA kit is to be used to detect and quantify protein levels of endogenous S100A8/S100A9. The assay recognizes human S100A8/S100A9. An antibody specific for S100A8/S100A9 has been pre-coated onto the microwells. The S100A8/S100A9 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human S100A8/S100A9 is added to detect the captured human S100A8/S100A9 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm .
| Product name | Human S100A8/S100A9 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Cell culture supernatants, Urine, Saliva, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 11.8 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6279 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 79%- 123% |
| Cell culture supernatants | 92% | 81%- 106% |
| Human milk | 89% | 76%- 122% |
| Urine | 80% | 76%- 96% |
| Saliva | 93% | 78%- 119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,816.5 | 76.8 | 4.2 |
| 2 | 20 | 413.2 | 18.6 | 4.5 |
| 3 | 20 | 92.4 | 6.1 | 6.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,994.1 | 126.3 | 6.3 |
| 2 | 24 | 457.5 | 38.9 | 8.5 |
| 3 | 24 | 115.4 | 11.1 | 9.7 |
Background Information
S100A8 and S100A9 (also known as MRP8 and MRP14, respectively) are Ca2+ binding proteins belonging to the S100 family. Calprotectin, is a heterodimeric calcium-binding protein consisting of S100A8 and S100A9 subunits. They are expressed in a wide variety of cell types and are abundant in myeloid cells such as: neutrophils, monocytes, keratinocytes and early differentiation states of macrophages. S100A8 and S100A9 have already been confirmed to play a decisive role in the development of inflammation. Elevated levels of S100A8/S100A9 were detected in inflammation, neoplastic tumor cells and various human cancers.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 60B8AG, CAGA, Calgranulin A, Calprotectin L1L subunit, CFAG, CGLA, CP 10, Cystic fibrosis antigen, L1Ag, MA387, MIF, MRP 8, MRP8, NIF, P8, Protein S100 A8, S100A8, Urinary stone protein band A |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cell Death Dis Gasdermin E promotes translocation of p65 and c-jun into nucleus in keratinocytes for progression of psoriatic skin inflammation | ||
J Exp Clin Cancer Res S100A9+CD14+ monocytes contribute to anti-PD-1 immunotherapy resistance in advanced hepatocellular carcinoma by attenuating T cell-mediated antitumor function | ||
Cell Rep Med Alarmin S100A8 imparts chemoresistance of esophageal cancer by reprogramming cancer-associated fibroblasts | ||
Front Physiol Impact of a tailored exercise regimen on physical capacity and plasma proteome profile in post-COVID-19 condition | ||
Respir Res Extracellular vesicle-bound S100A8/A9 is differentially expressed in septic shock and prompts acute lung injury | ||
Front Mol Biosci Expression and clinical significance of S100A8/9 in adults with secondary phagocytic lymphohistiocytosis |
