SARS-CoV-2 N protein SARS-CoV-2 N protein ELISA Kit
Cat no : KE30007
Synonyms
nucleocapsid phosphoprotein, SARS-COV-2 nucleocapsid phosphoprotein, N protein, sars cov 2
Validation Data Gallery
Product Information
KE30007 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SARS-CoV-2 N protein ELISA kit is to be used to detect and quantify protein levels of endogenous SARS-CoV-2 N protein. The assay recognizes human SARS-CoV-2 N protein. An antibody specific for SARS-CoV-2 N protein has been pre-coated onto the microwells. The SARS-CoV-2 N protein in samples is captured by the coated antibody after incubation, Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for SARS-CoV-2 N is added to detect the captured SARS-CoV-2 N protein. followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | SARS-CoV-2 N protein SARS-CoV-2 N protein ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 38 pg/mL |
| Range | 375-6000 pg/mL |
| Reactivity | SARS-CoV-2 N protein |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 43740575 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 116% | 95%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 3011.9 | 119.3 | 4.0 |
| 2 | 20 | 693.7 | 38.5 | 5.5 |
| 3 | 20 | 327.1 | 22.5 | 6.9 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2936.3 | 76.7 | 2.6 |
| 2 | 24 | 682.9 | 30.1 | 4.4 |
| 3 | 24 | 326.6 | 22.9 | 7.0 |
Background Information
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | nucleocapsid phosphoprotein, SARS-COV-2 nucleocapsid phosphoprotein, N protein, sars cov 2 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Appl Nano Mater Lethal Interactions of SARS-CoV-2 with Graphene Oxide: Implications for COVID-19 Treatment | ||
BMJ Open Determining the incidence, risk factors and biological drivers of irritable bowel syndrome (IBS) as part of the constellation of postacute sequelae of SARS-CoV-2 infection (PASC) outcomes in the Arizona CoVHORT-GI: a longitudinal cohort study | ||
