Human P-selectin ELISA Kit
Cat No : KE00064
Synonyms
CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selecin, SELP
Validation Data Gallery
Product Information
KE00064 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SELP ELISA kit is to be used to detect and quantify protein levels of endogenous SELP. The assay recognizes human SELP. An antibody specific for SELP has been pre-coated onto the microwells. The SELP protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for SELP is added to detect the captured SELP protein. For signal development, Streptavidin-HRP is added is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human P-selectin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 4.7 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6403 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 97% | 78%-107% |
| Human plasma | 100% | 85%-109% |
| Cell culture supernatants | 95% | 84%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,878.5 | 46.1 | 2.5 |
| 2 | 20 | 483.9 | 14.9 | 3.1 |
| 3 | 20 | 118.6 | 6.2 | 5.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,983.3 | 75.9 | 3.8 |
| 2 | 24 | 479.6 | 18.6 | 3.9 |
| 3 | 24 | 104.6 | 6.4 | 6.1 |
Background Information
SELP, also named as P-selectin, GMP-140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. Soluble P-selectin is produced by platelets and endothelial cells and circulates in normal plasma.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD62, CD62P, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, P-selecin, SELP |
Publications
| Species | Sample Type | Title |
|---|---|---|
Int J Mol Sci Decreased Platelet Specific Receptor Expression of P-Selectin and GPIIb/IIIa Predict Future Non-Surgical Bleeding in Patients after Left Ventricular Assist Device Implantation | ||
Intern Med Nocturnal Hypoxemia and High Circulating TNF-α Levels in Chronic Thromboembolic Pulmonary Hypertension. | ||
Platelets High plasma soluble CLEC-2 level predicts oxygen therapy requirement in patients with COVID-19 |