Human SERPINA1 ELISA Kit
Cat no : KE00178
Synonyms
a1-antitrypsin, Alpha-1-antitrypsin, Serpin A1, A1A, A-1-Antitrypsin
Validation Data Gallery
Product Information
KE00178 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA1 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA1. The assay recognizes human SERPINA1. An antibody specific for SERPINA1 has been pre-coated onto the microwells. The SERPINA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for SERPINA1 is added to detect the captured SERPINA1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human SERPINA1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.20 ng/mL |
| Range | 1.56 - 100 ng/mL , 6.25 - 400 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5265 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 81%-118% |
| Human urine | 88% | 77%-104% |
| cell culture supernatants | 101% | 90%-116% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 9.7 | 1.0 | 10 |
| 2 | 20 | 48.9 | 3.9 | 8.1 |
| 3 | 20 | 210.1 | 18.6 | 8.8 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 10.4 | 1.1 | 10.1 |
| 2 | 24 | 41.6 | 4.1 | 9.8 |
| 3 | 24 | 196.8 | 13.2 | 6.7 |
Background Information
SERPINA1 is the archetypal member of the Serpin superfamily of the serine protease inhibitors. It is an acute phase glycoprotein mainly (by 80%) synthesized in human liver and is a major blood protein after albumin and the Immunoglobulins. SERPINA1 plays a key antiinflammatory role to protect tissues against proteolytic digestion by elastase secreted by neutrophils. In patients with carcinomas, it was reported that SERPINA1 promotes invasive and metastatic capacity in lung cancer, gastric cancer, and colorectal carcinomas.Several studies demonstrated that higher levels of SERPINA1 correlate with more advanced cancer stages. Defects in this gene can cause emphysema or liver disease.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | a1-antitrypsin, Alpha-1-antitrypsin, Serpin A1, A1A, A-1-Antitrypsin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Cells Seasonal Influences on Human Placental Transcriptomes Associated with Spontaneous Preterm Birth | ||
Inflammation Alpha-1 Antitrypsin Mediates Spontaneous Resolution of Acute Gouty Inflammation Via Inhibiting Caspase 3/GSDME-dependent Macrophage Pyroptosis. |
