Human TF ELISA Kit
Sensitivity
0.6 ng/mL
Range
1.56-100 ng/mL
Reactivity
Human
Cat no : KE00046
Synonyms
Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin
Validation Data Gallery
Product Information
KE00046 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TF ELISA kit is to be used to detect and quantify protein levels of endogenous TF. The assay recognizes human TF. An antibody specific for TF has been pre-coated onto the microwells. The TF protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for TF is added to detect the captured TF protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TF ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.6 ng/mL |
| Range | 1.56-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7018 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Urine | 88% | 79%-97% |
| Human plasma | 103% | 81%-126% |
| Cell culture supernatants | 102% | 90%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 111.4 | 5.6 | 5.0 |
| 2 | 20 | 18.4 | 0.8 | 4.2 |
| 3 | 20 | 4.5 | 0.2 | 5.3 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 101.3 | 8.2 | 8.1 |
| 2 | 24 | 17.9 | 1.3 | 7.2 |
| 3 | 24 | 4.3 | 0.3 | 7.5 |
Background Information
Serotransferrin (TF) is a 77 kDa secreted protein which is also named as transferrin, siderophilin, beta-1 metal-binding globulin and belongs to a family of homologous iron-binding glycoproteins that encompasses lactoferrin (found both intracellular and in secretions, including milk), melanotrasferrin (present on melanoma cells) and ovotransferrin (present in egg white). TF is a multi-function protein with a primary role in transporting iron in a safe, redox-inactive state from absorption to utilization or storage sites around the body. The association of Tf with the immune system derives from its ability to restrict serum free-iron levels, creating low-iron environments where the infection capacity of pathogenic microorganisms is limited. TF is expressed predominantly in the liver and secreted in plasma, but lower amounts can be synthesized in other tissues such as the brain and the testis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, transferrin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
