Human TIMP2 ELISA Kit
Validation Data Gallery
Product Information
KE00162 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TIMP2 ELISA kit is to be used to detect and quantify protein levels of endogenous TIMP2. The assay recognizes human TIMP2. An antibody specific for TIMP2 has been pre-coated onto the microwells. The TIMP2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TIMP2 is added to detect the captured human TIMP2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TIMP2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Urine, Saliva |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7077 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 99% | 72%-119% |
| Cell culture supernatants | 97% | 90%-116% |
| Urine | 107% | 98%-117% |
| Saliva | 103% | 94%-113% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 987.5 | 40.1 | 4.1 |
| 2 | 20 | 255.8 | 7.9 | 3.1 |
| 3 | 20 | 84.9 | 3.2 | 3.8 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 971.0 | 31.5 | 3.2 |
| 2 | 24 | 262.8 | 7.5 | 2.8 |
| 3 | 24 | 88.7 | 2.4 | 2.7 |
Background Information
TIMP2, a 21-kDa protein, is a member of the family of matrix metalloproteinase inhibitors, which contains four members (TIMP1, TIMP2, TIMP3, and TIMP4). Tissue inhibitors of metalloproteinases (TIMPs) are multifaceted molecules that exhibit properties beyond their classical proteinase inhibitory function. TIMP-2 is the only TIMP functioning as both an MMP inhibitor and an activator, because it can interact with membrane type 1 MMP and pro-MMP2 to facilitate the activation of pro-MMP2 to its active form. TIMP2 can regulate many physiological progression such as tumor growth, metastasis, and angiogenesis through matrix metalloproteinases (MMPs). TIMP2 knockout resulted in constitutive MMP9 activation abundantly and adverse MI remodeling in vivo.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CSC 21K, Metalloproteinase inhibitor 2, TIMP 2, TIMP2 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Crit Care A translational study of Galectin-3 as an early biomarker and potential therapeutic target for ischemic-reperfusion induced acute kidney injury. |