Human TNFRSF1B ELISA Kit
Sensitivity
4.5 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Cat No : KE00224
Synonyms
CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B
Validation Data Gallery
Product Information
KE00224 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TNFRSF1B ELISA kit is to be used to detect and quantify protein levels of endogenous TNFRSF1B. The assay recognizes human TNFRSF1B. An antibody specific for TNFRSF1B has been pre-coated onto the microwells. The TNFRSF1B protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human TNFRSF1B is added to detect the captured human TNFRSF1B protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFRSF1B ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell cuture supernatants |
| Assay type | Sandwich |
| Sensitivity | 4.5 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7133 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 78%-113% |
| Cell cuture supernatants | 83% | 71%-102% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 911.9 | 68.7 | 7.5 |
| 2 | 20 | 227.6 | 8.8 | 3.9 |
| 3 | 20 | 56.6 | 4.7 | 8.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 817.2 | 67.0 | 8.2 |
| 2 | 24 | 211.0 | 15.0 | 7.1 |
| 3 | 24 | 59.0 | 5.0 | 8.4 |
Background Information
TNFRSF1B, also known as TNFR2 or CD120b or p75, is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1A. Tumor necrosis factor (TNF, TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through TNFRSF1A and TNFRSF1B. TNFRSF1A is widely expressed, whereas TNFRSF1B exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells. Both TNFRSF1A and TNFRSF1B can also exist in soluble forms, probably derived by proteolytic cleavage from the cell surface forms. Various defects in the TNFRSF1B pathway, due to polymorphisms in the TNFRSF1B gene, upregulated expression of TNFRSF1B and TNFRSF1B shedding, have been implicated in the pathology of several autoimmune disorders.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B |