Human Thrombospondin 1 ELISA Kit
Cat no : KE00249
Synonyms
THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1
Validation Data Gallery
Product Information
KE00249 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Thrombospondin 1 ELISA kit is to be used to detect and quantify protein levels of endogenous Thrombospondin 1 . The assay recognizes human Thrombospondin 1 . An antibody specific for Thrombospondin 1 has been pre-coated onto the microwells. The Thrombospondin 1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Thrombospondin 1 is added to detect the captured human Thrombospondin 1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Thrombospondin 1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Saliva, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.002 ng/mL |
| Range | 0.137-100 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7057 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 81% | 70%-101% |
| Cell culture supernatants | 88% | 72%-100% |
| Saliva | 84% | 70%-117% |
| Human milk | 88% | 77%-114% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 44.43 | 4.17 | 9.4 |
| 2 | 20 | 3.71 | 0.31 | 8.3 |
| 3 | 20 | 0.31 | 0.03 | 9.6 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 36.57 | 3.67 | 10.0 |
| 2 | 24 | 3.66 | 0.20 | 5.5 |
| 3 | 24 | 0.34 | 0.03 | 8.3 |
Background Information
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Redox Biol Endothelial transferrin receptor 1 contributes to thrombogenesis through cascade ferroptosis | ||
Ann Hematol Lycorine inhibits the proliferation of acute myeloid leukemia cells by upregulating the expression of THBS1. |
