Human Total IL-18 ELISA Kit
Validation Data Gallery
Product Information
KE00193 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total IL-18 ELISA kit is to be used to detect and quantify protein levels of endogenous Total IL-18. The assay recognizes human Total IL-18. An antibody specific for Total IL-18 has been pre-coated onto the microwells. The Total IL-18 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human Total IL-18 is added to detect the captured human Total IL-18 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total IL-18 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum,Plasma 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3606 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 109% | 98%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 475.7 | 22.1 | 4.7 |
| 2 | 20 | 112.5 | 3.6 | 3.2 |
| 3 | 20 | 27.3 | 1.4 | 5.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 506.3 | 49.1 | 9.7 |
| 2 | 24 | 114.3 | 9.6 | 8.4 |
| 3 | 24 | 28.0 | 1.5 | 5.5 |
Background Information
IL-18 is a member of IL-1 superfamily of cytokines. IL-18 is expressed by many human lymphoid and nonlymphoid cells, and has an important role in inflammatory processes. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL-18 synergistically with IL-12 contributes to Th1 differentiation and, therefore, is important in host defense mechanisms against intracellular bacteria, viruses, and fungi. The IL-18 cytokine increased expression of vascular cell adhesion molecule-1(VCAM1) and the adherence of melanoma cells. IL-18 plays a major role in atherosclerotic plaque destabilization leading to acute ischemic syndromes. IL-18 negatively regulate NAFLD/NASH progression, as well as multiple aspects of metabolic syndrome via modulation of the gut microbiota. KE00193 detects the region corresponding to the mature form of IL-18 and is not specific for pro-IL-18.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IL18, IL-18, Pre-IL18, Iboctadekin, IFN-gamma-inducing factor |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Nanobiotechnology A bioadhesive antioxidant dual-crosslinked hydrogel mitigates endothelial pyroptosis to enhance ischemic flap survival. | ||
Theranostics Discovery of indole analogue Tc3 as a potent pyroptosis inducer and identification of its combination strategy against hepatic carcinoma | ||
Adv Sci (Weinh) Radiation-Induced Tumor-Intrinsic LTβR N-Glycosylation Suppresses Pyroptosis Through TRIM28-Mediated PCBP2 SUMOylation to Promote Gastric Cancer Radioresistance | ||
Research (Wash D C) Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation | ||
Int J Biol Sci Cisplatin Induces Pyroptosis via Activation of MEG3/NLRP3/caspase-1/GSDMD Pathway in Triple-Negative Breast Cancer. | ||
Cell Death Discov Microtubule associated protein 4 (MAP4) phosphorylation reduces cardiac microvascular density through NLRP3-related pyroptosis. |
