Human Total MMP-1 ELISA Kit
Cat no : KE00223
Synonyms
CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1
Validation Data Gallery
Product Information
KE00223 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-1. The assay recognizes human MMP-1. An antibody specific for MMP-1 has been pre-coated onto the microwells. The MMP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human MMP-1 is added to detect the captured human MMP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Total MMP-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 7.7 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 4312 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 96% | 81%-108% |
| Cell culture supernatants | 85% | 77%-95% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,037.2 | 28.6 | 2.8 |
| 2 | 20 | 244.1 | 5.6 | 2.3 |
| 3 | 20 | 58.3 | 1.9 | 3.3 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,050.6 | 23.5 | 2.2 |
| 2 | 24 | 244.8 | 6.9 | 2.8 |
| 3 | 24 | 57.3 | 2.9 | 5.1 |
Background Information
Matrix metalloproteinase 1 (MMP1), which is also known as interstitial collagenase and fibroblast collagenase is a member of the matrix metalloproteinases (MMPs) family . Proteins in this family mainly participate in the breakdown of extracellular matrix both in normal physiological processes and disease processes. Several factors contribute to the expression of MMP1, including endogenous factors, such as polymorphisms and epigenetic regulation in the promoter region of MMP1, as well as exogenous factors, such as the tumor microenvironment. A variety of MMP1 promoter genotypic polymorphisms have been reported in various ethnic populations, and their contributions to different disease risks have been explored. In the tumor microenvironment, a variety of inflammatory factors, including interleukin-8 (IL-8), IL-1β and tumor necrosis factor-α (TNF-α), have been reported to induce MMP1 expression in cancer cells. (PMID: 31115512, PMID: 28334049)
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CLG, CLGN, Fibroblast collagenase, Interstitial collagenase, Matrix metalloproteinase 1, MMP 1, MMP1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
ACS Omega Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells | ||
J Cosmet Dermatol The Reduction of Skin Photodamage by the Ectoine-Thermus thermophilus Cofermentation Products | ||
Biomolecules Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies | ||
