Human Total MMP-3 ELISA Kit

Sensitivity

18.4 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat No : KE00160

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Synonyms

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, MMP-3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1



Product Information

KE00160 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Total MMP3 ELISA kit is to be used to detect and quantify protein levels of endogenous Total MMP3. The assay recognizes human Total MMP3. An antibody specific for Total MMP3 has been pre-coated onto the microwells. The Total MMP3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for Total MMP3 is added to detect the captured Total MMP3 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human Total MMP-3 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 18.4 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 4314

Recovery

Sample TypeAverageRange
Human serum98%89%-103%
Cell culture supernatants96%78%-117%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12060.65.28.6
220245.420.48.3
320954.743.74.6

InterAssay

Samplenmean ( pg/mL)SDCV%
12457.85.39.2
224201.812.56.2
324870.957.06.5

Background Information

MMP-3 (matrix metallopeptidase 3), also named as Stromelysin 1 or STR1, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis and tissue remodelling and in many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fbronectin, laminin, IGFBP-3, serpins, and IL-1β.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, MMP-3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Gut Microbes

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway

Authors - Jian-Wei Zhang
humancell culture supernatants

Aging Cell

Activation of epidermal growth factor receptor signaling mediates cellular senescence induced by certain pro-inflammatory cytokines.

Authors - Dongsheng Shang
humantissue homogenates

EBioMedicine

Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis

Authors - Zhongyang Lv
humancell culture supernatants

Int Immunopharmacol

CX-4945 inhibits fibroblast-like synoviocytes functions through the CK2-p53 axis to reduce rheumatoid arthritis disease severity

Authors - Yanping Luo
humancell culture supernatants

ACS Omega

Evaluation of the Effects of E-Cigarette Aerosol Extracts and Tobacco Cigarette Smoke Extracts on Human Gingival Epithelial Cells

Authors - Le Su