Human UCH-L1 ELISA Kit
Cat no : KE00211
Synonyms
Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1
Validation Data Gallery
Product Information
KE00211 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The UCH-L1 ELISA kit is to be used to detect and quantify protein levels of endogenous UCH-L1. The assay recognizes human UCH-L1. An antibody specific for UCH-L1 has been pre-coated onto the microwells. The UCH-L1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for UCH-L1 is added to detect the captured UCH-L1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human UCH-L1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell Lysates 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 0.02 ng/mL |
| Range | 0.156-10 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7345 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human Serum | 92% | 84%-99% |
| Cell Lysates | 104% | 72%-127% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 4.58 | 0.16 | 3.4 |
| 2 | 20 | 1.14 | 0.03 | 2.9 |
| 3 | 20 | 0.30 | 0.02 | 5.9 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 4.54 | 0.22 | 4.9 |
| 2 | 24 | 1.02 | 0.03 | 3.1 |
| 3 | 24 | 0.23 | 0.02 | 8.6 |
Background Information
Ubiquitin C-terminal hydrolase 1 (UCHL1) , previously known as PGP9.5, is found primarily in neurons and neuroendocrine cells with a very high concentration in dendrites and perikarya. UCHL1 is a more recently recognized biomarker of neuronal injury. UCHL1 could be detected in the CSF and serum with a half-life of 7-9 hours after severe traumatic brain injury. Increased plasma concentrations of UCHL1 therefore reflect increased blood brain barrier permeability and destruction of neurons.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Neuron cytoplasmic protein 9.5, PARK5, PGP 9.5, PGP9.5, Ubiquitin thioesterase L1, Uch L1, UCHL1 |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
J Clin Lab Anal Serum ubiquitin C-terminal hydrolase L1 predicts cognitive impairment in patients with acute organophosphorus pesticide poisoning. | ||
Int J Hematol Plasma ubiquitin C-terminal hydrolase-L1 (UCH-L1) level as a blood biomarker of neurological damage after allogeneic hematopoietic cell transplantation |
