Human t-Plasminogen activator ELISA Kit
Cat no : KE00180
Synonyms
Alteplase, DKFZp686I03148, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, TPA
Validation Data Gallery
Product Information
KE00180 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The t-Plasminogen activator ELISA kit is to be used to detect and quantify protein levels of endogenous t-Plasminogen activator. The assay recognizes human t-Plasminogen activator. An antibody specific for t-Plasminogen activator has been pre-coated onto the microwells. The t-Plasminogen activator protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for t-Plasminogen activator is added to detect the captured t-Plasminogen activator protein. For signal development, horseradish peroxidase (HRP)-conjugated is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human t-Plasminogen activator ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, cell culture supernatants 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 8.9 pg/mL |
| Range | 156.3 - 10000 pg/mL, 78.1 - 5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5327 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 84% | 74%-93% |
| Plasma | 92% | 78%-125% |
| cell culture supernatants | 87% | 76%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,076.2 | 110.5 | 5.3 |
| 2 | 20 | 797.3 | 56.7 | 7.1 |
| 3 | 20 | 538.5 | 49.2 | 9.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,214.8 | 168.9 | 7.6 |
| 2 | 24 | 814.1 | 71.9 | 8.8 |
| 3 | 24 | 539.8 | 34.2 | 6.3 |
Background Information
Plasminogen activator, tissue (PLAT, synonyms: TPA, T-PA) is a tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein (33 kDa and 32 kDa). PLAT enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. tPA has 4 isoforms produced by alternative splicing with the MW of 63 kDa, 33 kDa, 57 kDa and 44 kDa.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Alteplase, DKFZp686I03148, plasminogen activator, tissue, PLAT, Reteplase, T PA, t plasminogen activator, TPA |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Molecules Bioevaluation of Pheretima vulgaris Antithrombotic Extract, PvQ, and Isolation, Identification of Six Novel PvQ-Derived Fibrinolytic Proteases. | ||
J Control Release Vascular endothelial cell-targeted mRNA delivery via synthetic lipid nanoparticles for venous thrombosis prevention |
