验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, sheep |
免疫原 | IDH1 fusion protein Ag2986 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | isocitrate dehydrogenase 1 (NADP+), soluble |
别名 | Cytosolic NADP-isocitrate dehydrogenase, EC:1.1.1.42, IDCD, IDH, IDH 1 |
计算分子量 | 414 aa, 47 kDa |
观测分子量 | 47 kDa |
GenBank蛋白编号 | BC012846 |
基因名称 | IDH1 |
Gene ID (NCBI) | 3417 |
RRID | AB_2123159 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O75874 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).
实验方案
Product Specific Protocols | |
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WB protocol for IDH1 antibody 12332-1-AP | Download protocol |
IHC protocol for IDH1 antibody 12332-1-AP | Download protocol |
IF protocol for IDH1 antibody 12332-1-AP | Download protocol |
IP protocol for IDH1 antibody 12332-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature IDH mutation impairs histone demethylation and results in a block to cell differentiation. | ||
Cancer Cell Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation. | ||
Cell Stem Cell PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs. |