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IDH1 Polyclonal antibody

IDH1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12332-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

Cytosolic NADP-isocitrate dehydrogenase, EC:1.1.1.42, IDCD, IDH, IDH 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, rat liver tissue, HeLa cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12332-1-AP targets IDH1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, sheep
免疫原 IDH1 fusion protein Ag2986 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 1 (NADP+), soluble
别名 Cytosolic NADP-isocitrate dehydrogenase, EC:1.1.1.42, IDCD, IDH, IDH 1
计算分子量 414 aa, 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC012846
基因名称 IDH1
Gene ID (NCBI) 3417
RRIDAB_2123159
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171). IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).

实验方案

Product Specific Protocols
WB protocol for IDH1 antibody 12332-1-APDownload protocol
IHC protocol for IDH1 antibody 12332-1-APDownload protocol
IF protocol for IDH1 antibody 12332-1-APDownload protocol
IP protocol for IDH1 antibody 12332-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

α-Ketoglutarate links p53 to cell fate during tumour suppression.

Authors - John P Morris
  • KD Validated
humanWB,IHC

Nature

IDH mutation impairs histone demethylation and results in a block to cell differentiation.

Authors - Lu Chao C
humanWB

Nature

Cancer-associated IDH1 mutations produce 2-hydroxyglutarate.

Authors - Dang Lenny L
humanWB

Nature

Quantitative flux analysis reveals folate-dependent NADPH production.

Authors - Jing Fan
humanWB

Cancer Cell

Leukemic IDH1 and IDH2 mutations result in a hypermethylation phenotype, disrupt TET2 function, and impair hematopoietic differentiation.

Authors - Maria E Figueroa
humanWB

Cell Stem Cell

PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs.

Authors - Zhexin Zhu
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