验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, human skeletal muscle tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15909-1-AP targets IDH3A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | IDH3A fusion protein Ag8662 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | isocitrate dehydrogenase 3 (NAD+) alpha |
别名 | NAD(+)-specific ICDH subunit alpha, Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial, IDH3, EC:1.1.1.41 |
计算分子量 | 366 aa, 40 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC021967 |
基因名称 | IDH3A |
Gene ID (NCBI) | 3419 |
RRID | AB_2123282 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P50213 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for IDH3A antibody 15909-1-AP | Download protocol |
IHC protocol for IDH3A antibody 15909-1-AP | Download protocol |
IF protocol for IDH3A antibody 15909-1-AP | Download protocol |
IP protocol for IDH3A antibody 15909-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk. | ||
EMBO J Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues. | ||
J Invest Dermatol Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata. | ||
Cell Physiol Biochem Energy Metabolism in the Bone is Associated with Histomorphometric Changes in Rats with Hyperthyroidism. |