验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, RAW 264.7 cells, THP-1 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human skin cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12604-1-AP targets IFIT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | IFIT2 fusion protein Ag3283 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | IFIT 2 |
| 别名 | IFI 54K, IFI 54, GARG 39, G10P2, CIG-42 |
| 计算分子量 | 484 aa, 56 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC032839 |
| 基因名称 | IFIT2 |
| Gene ID (NCBI) | 3433 |
| RRID | AB_2864734 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09913 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| IHC protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| IF protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| IP protocol for IFIT2 antibody 12604-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Immunol A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity. | ||
Nat Microbiol ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity. | ||
Nat Microbiol Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs. | ||
Nucleic Acids Res IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA. |
