- Featured Product
- KD/KO Validated
IFITM1 Polyclonal antibody
IFITM1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (2)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, K-562 cells |
| Positive IP detected in | ROS1728 cells |
| Positive IHC detected in | human lymphoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IFITM1 Ready-To-Use IHC Kit. IFITM1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11727-3-AP targets IFITM1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, goat, African green monkey |
| Immunogen | IFITM1 fusion protein Ag2320 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interferon induced transmembrane protein 1 (9-27) |
| Synonyms | 9 27, CD225, IFI17, IFITM1, Interferon induced protein 17, Leu 13 antigen, LEU13 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-17 kDa |
| GenBank Accession Number | BC000897 |
| Gene Symbol | IFITM1 |
| Gene ID (NCBI) | 8519 |
| RRID | AB_2122083 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13164 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM1(interferon induced transmembrane protein), also named DSPA2a or interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM1 antibody 11727-3-AP | Download protocol |
| IHC protocol for IFITM1 antibody 11727-3-AP | Download protocol |
| IP protocol for IFITM1 antibody 11727-3-AP | Download protocol |
| FC protocol for IFITM1 antibody 11727-3-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Mitotic progression following DNA damage enables pattern recognition within micronuclei. | ||
Nature Intrinsic retroviral reactivation in human preimplantation embryos and pluripotent cells.
| ||
Nat Immunol Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity. | ||
Mol Ther Type 1 Interferon Responses Underlie Tumor-Selective Replication of Oncolytic Measles Virus. | ||
Nat Commun IFITM3 restricts virus-induced inflammatory cytokine production by limiting Nogo-B mediated TLR responses | ||
Cell Rep IFITM Proteins Restrict HIV-1 Infection by Antagonizing the Envelope Glycoprotein.
|
