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验证数据展示

  • WB analysis using 66081-1-Ig (same clone as 66081-1-PBS)

    WB analysis using 66081-1-Ig (same clone as 66081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

    IHC staining of human liver cancer using 66081-1-Ig (same clone as 66081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66081-1-Ig (IFITM2/3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFITM2/3 antibody included
  • IF Staining of HeLa using 66081-1-Ig (same clone as 66081-1-PBS)

    IF Staining of HeLa using 66081-1-Ig (same clone as 66081-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using IFITM2/3 antibody (66081-1-Ig, Clone: 2E8D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66081-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IFITM2/3 Monoclonal antibody, PBS Only

IFITM2/3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66081-1-PBS

产品说明书

CloneNo. 2E8D12

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

IFITM3, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, DSPA2b, 2E8D12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IFITM2/3 Ready-To-Use IHC Kit. IFITM2/3 primary antibody included.

产品信息

66081-1-PBS targets IFITM2/3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 IFITM2/3 fusion protein Ag17863 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 interferon induced transmembrane protein 3 (1-8U)
别名 IFITM3, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, DSPA2b, 2E8D12
观测分子量 15-20 kDa
GenBank蛋白编号BC070243
基因名称 IFITM3
Gene ID (NCBI) 10410
RRIDAB_11182821
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.