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IFNAR2 Polyclonal antibody

IFNAR2 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 10522-1-AP

Print datasheet

Synonyms

IFN-alpha binding protein, IFNABR, IFN R 2, IFN R, IFN alpha/beta receptor 2

验证数据展示

  • WB analysis using 10522-1-AP

    WB analysis using 10522-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10522-1-AP (IFNAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 10522-1-AP

    FC experiment of MCF-7 using 10522-1-AP

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug IFNAR2 Polyclonal antibody (10522-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10522-1-AP targets IFNAR2 in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human
Cited Reactivityhuman, pig
Immunogen IFNAR2 fusion protein Ag0191 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon (alpha, beta and omega) receptor 2
Synonyms IFN-alpha binding protein, IFNABR, IFN R 2, IFN R, IFN alpha/beta receptor 2
Calculated Molecular Weight 331 aa, 37 kDa
Observed Molecular Weight 58-70 kDa
GenBank Accession NumberBC002793
Gene Symbol IFNAR2
Gene ID (NCBI) 3455
RRIDAB_10694421
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48551
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFNAR2 antibody 10522-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

Degradation of WTAP blocks antiviral responses by reducing the m 6 A levels of IRF3 and IFNAR1 mRNA

Authors - Yong Ge
View Article
humanWB,IP

J Virol

African swine fever virus pH240R enhances viral replication via inhibition of the type I IFN signaling pathway

Authors - Guangqiang Ye
View Article
humanWB

J Virol

Herpes simplex virus 1 UL36USP antagonizes type I IFN-mediated antiviral innate immunity.

Authors - Hui Yuan
View Article
pigWB

PLoS Pathog

African swine fever virus pB318L, a trans-geranylgeranyl-diphosphate synthase, negatively regulates cGAS-STING and IFNAR-JAK-STAT signaling pathways

Authors - Xiaohong Liu
View Article