IFT122 Polyclonal antibody

IFT122 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19304-1-AP

Print datasheet

Synonyms

IFT122, SPG, WDR10, WDR10p, WDR140



经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human brain tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19304-1-AP targets IFT122 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse
Immunogen IFT122 fusion protein Ag6860 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 122 homolog (Chlamydomonas)
Synonyms IFT122, SPG, WDR10, WDR10p, WDR140
Calculated Molecular Weight 142 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC004238
Gene Symbol IFT122
Gene ID (NCBI) 55764
RRIDAB_2714184
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBG6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFT122 antibody 19304-1-APDownload protocol
IHC protocol for IFT122 antibody 19304-1-APDownload protocol
IF protocol for IFT122 antibody 19304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell

Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision.

Authors - Siew Cheng Phua
mouseWB,IF

Elife

A WDR35-dependent coat protein complex transports ciliary membrane cargo vesicles to cilia.

Authors - Tooba Quidwai
humanWB

Hum Mol Genet

Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis.

Authors - Mariko Takahara