• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

IFT88 Monoclonal antibody, PBS Only

IFT88 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A4G5

Cat no : 60227-1-PBS

Print datasheet

Synonyms

hTg737, IFT88, TG737, TPR repeat protein 10, TTC10

验证数据展示

  • IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

    IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

    IHC staining of human kidney using 60227-1-Ig (same clone as 60227-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 60227-1-Ig(IFT88 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

    WB analysis of human testis using 60227-1-Ig (same clone as 60227-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 60227-1-Ig (IFT88 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 60227-1-Ig (same clone as 60227-1-PBS)

    IP experiment of PC-3 using 60227-1-Ig (same clone as 60227-1-PBS)

    IP result of anti-IFT88 (IP:60227-1-Ig, 5ug; Detection:60227-1-Ig 1:800) with PC-3 cells lysate 1040ug. This data was developed using the same antibody clone with 60227-1-PBS in a different storage buffer formulation.

产品信息

60227-1-PBS targets IFT88 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IFT88 fusion protein Ag4980 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Synonyms hTg737, IFT88, TG737, TPR repeat protein 10, TTC10
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 88-95 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13099
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.