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human IgM Polyclonal antibody

human IgM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11016-1-AP

Print datasheet

Synonyms

human IgM, IGHM, IgM, MU, VH

验证数据展示

  • IHC staining of human tonsillitis using 11016-1-AP

    IHC staining of human tonsillitis using 11016-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included
  • IHC staining of human tonsillitis using 11016-1-AP

    IHC staining of human tonsillitis using 11016-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with human IgM antibody included
  • WB analysis of human plasma using 11016-1-AP

    WB analysis of human plasma using 11016-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 11016-1-AP (IGHM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy human IgM Ready-To-Use IHC Kit. human IgM primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11016-1-AP targets human IgM in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen human IgM fusion protein Ag1459 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immunoglobulin heavy constant mu
Synonyms human IgM, IGHM, IgM, MU, VH
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC009851
Gene Symbol IGHM
Gene ID (NCBI) 3507
RRIDAB_593462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgM (~75 kDa).

实验方案

Product Specific Protocols
WB protocol for human IgM antibody 11016-1-APDownload protocol
IHC protocol for human IgM antibody 11016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Proteomics

Tear dynamics testing and quantitative proteomics analysis in patients with chronic renal failure.

Authors - Rong-Rong Zong
View Article