验证数据展示
经过测试的应用
| Positive IHC detected in | mouse pancreas tissue, human pancreas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human pancreas tissue, mouse pancreas tissue, rat pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15848-1-AP targets INS in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IHC, IF, ELISA |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 | INS fusion protein Ag8630 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | INS |
| 别名 | insulin |
| 计算分子量 | 110 aa, 12 kDa |
| GenBank蛋白编号 | BC005255 |
| 基因名称 | INS |
| Gene ID (NCBI) | 3630 |
| RRID | AB_10597100 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01308 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for INS antibody 15848-1-AP | Download protocol |
| IF protocol for INS antibody 15848-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Protoc Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro. | ||
Autophagy Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo | ||
Theranostics Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus. | ||
ACS Appl Mater Interfaces Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets. |
