INS Polyclonal antibody

INS Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15848-1-AP

Print datasheet

Synonyms

ILPR, INS, IRDN



经过测试的应用

Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman pancreas tissue, mouse pancreas tissue, rat pancreas tissue
Planning an IHC experiment? We recommend our IHCeasy Insulin Ready-To-Use IHC Kit. Insulin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15848-1-AP targets INS in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IF-P, ELISA Application Description
Cited ApplicationsIHC, IF, ELISA
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen INS fusion protein Ag8630 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name INS
Synonyms ILPR, INS, IRDN
Calculated Molecular Weight 110 aa, 12 kDa
GenBank Accession NumberBC005255
Gene Symbol INS
Gene ID (NCBI) 3630
RRIDAB_10597100
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01308
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

INS is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. INS is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form INS, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in INS results in type 1 diabetes mellitus.

实验方案

Product Specific Protocols
IHC protocol for INS antibody 15848-1-APDownload protocol
IF protocol for INS antibody 15848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell

Long-Term Expansion of Pancreatic Islet Organoids from Resident Procr+ Progenitors.

Authors - Daisong Wang
mouseIF

Nat Protoc

Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro.

Authors - Jingqiang Wang
mouseIF

Autophagy

Secretory autophagy promotes RAB37-mediated insulin secretion under glucose stimulation both in vitro and in vivo

Authors - Shan-Ying Wu
mouseIHC

Cell Rep

Spatiotemporal role of SETD2-H3K36me3 in murine pancreatic organogenesis

Authors - Ping Lu
mouseIHC

Theranostics

Circular RNA circPPM1F modulates M1 macrophage activation and pancreatic islet inflammation in type 1 diabetes mellitus.

Authors - Caiyan Zhang
mouseIF

ACS Appl Mater Interfaces

Localized Controlled Release of Bilirubin from β-Cyclodextrin-Conjugated ε-Polylysine To Attenuate Oxidative Stress and Inflammation in Transplanted Islets.

Authors - Qing Yao