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IRF3 Monoclonal antibody, PBS Only

IRF3 Monoclonal Antibody for WB,IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1E6G8

Cat No : 66670-1-PBS

Print datasheet

Synonyms

interferon regulatory factor 3, IRF 3, IRF3

验证数据展示

  • WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

    WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

    IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IRF3 antibody included
  • WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

    WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

产品信息

66670-1-PBS targets IRF3 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,IHC,ELISA Application Description
Tested Reactivity Human
Immunogen IRF3 fusion protein Ag27223 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon regulatory factor 3
Synonyms interferon regulatory factor 3, IRF 3, IRF3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14653
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.