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  • Featured Product
  • KD/KO Validated

MUM1/IRF4 Polyclonal antibody

MUM1/IRF4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11247-2-AP

Print datasheet

Synonyms

IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4

验证数据展示

  • IHC staining of human thymus using 11247-2-AP

    IHC staining of human thymus using 11247-2-AP

    Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IRF4 antibody included
  • WB analysis using 11247-2-AP

    WB analysis using 11247-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11247-2-AP (MUM1/IRF4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse spleen using 11247-2-AP

    IP experiment of mouse spleen using 11247-2-AP

    IP result of anti-MUM1/IRF4 (IP:11247-2-AP, 3ug; Detection:11247-2-AP 1:500) with mouse spleen tissue lysate 4000ug.

  • IF Staining of RAW 264.7 using 11247-2-AP

    IF Staining of RAW 264.7 using 11247-2-AP

    Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using MUM1/IRF4 antibody (11247-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse spleen tissue, mouse lung tissue, mouse thymus tissue, Raji cells, RAW264.7, rat spleen tissue
Positive IP detected inmouse spleen tissue
Positive IHC detected inhuman thymus tissue, human lymphoma tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy IRF4 Ready-To-Use IHC Kit. IRF4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11247-2-AP targets MUM1/IRF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MUM1/IRF4 fusion protein Ag1762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name IRF 4
Synonyms IRF4, Lymphocyte-specific interferon regulatory factor, LSIRF, IRF-4, IRF 4
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015752
Gene Symbol IRF4
Gene ID (NCBI) 3662
ENSEMBL Gene IDENSG00000137265
RRIDAB_2264940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15306
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IRF4 is a member of the IRF family of transcription factors, expressed in most cell types of the immune system. IRF4 is a transcription factor essential for the development of T helper-2 (Th2) cells, IL17-producing Th17 cells, and IL9-producing Th9 cells, as well as dendritic cell (DC). It binds to and activates the ISRE of the MHC class I promoter, and may have a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.

实验方案

Product Specific Protocols
WB protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IHC protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IF protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
IP protocol for MUM1/IRF4 antibody 11247-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cancer Commun (Lond)

Targeting autophagy overcomes cancer-intrinsic resistance to CAR-T immunotherapy in B-cell malignancies

Authors - Lu Tang
View Article
human,mouseWB

Diabetes

Obese Skeletal Muscle-Expressed Interferon Regulatory Factor 4 Transcriptionally Regulates Mitochondrial Branched-Chain Aminotransferase Reprogramming Metabolome.

Authors - Ting Yao
  • KD Validated
View Article
mouseWB

Nanotoxicology

Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis.

Authors - Jie Dong
View Article
humanIHC

Diabetes Res Clin Pract

fMaggot treatment promotes healing of diabetic foot ulcer wounds possibly by upregulating Treg levels.

Authors - Jie Zhang
View Article
mouseIHC

Int Immunopharmacol

IRF-4 deficiency reduces inflammation and kidney fibrosis after folic acid-induced acute kidney injury.

Authors - Meixin Chen
  • KO Validated
View Article
mouseWB,IHC

Mediators Inflamm

MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression.

Authors - Aijun Jia
View Article