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Atlantic Blue™ Mouse IgG1 Isotype Control (MOPC-21)

IgG1 Isotype Control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Applications

FC

Conjugate

Atlantic Blue™ Fluorescent Dye

CloneNo.

MOPC-21

Cat no : AB-65124

Print datasheet

Synonyms

IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control

验证数据展示

  • FC experiment of human PBMCs using AB-65124

    FC experiment of human PBMCs using AB-65124

    1X10^6 human PBMCs were surface stained with 0.2 ug Atlantic Blue™ Anti-Mouse IgG1 Isotype Control (AB-65124, Clone:MOPC-21) (red), unstained (blue), or Atlantic Blue™ Anti-Human CD3 (UCHT1) (green). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected in

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

AB-65124 targets IgG1 Isotype Control in FC applications and shows reactivity with samples.

Tested Applications FC Application Description
Tested Reactivity
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant gamma 1 (G1m marker)
Synonyms IgG1, IgG1 Isotype Control, mouse IgG1, Mouse IgG1 Isotype Control
Gene Symbol IgG1 Isotype Control
Gene ID (NCBI) 16017
Conjugate Atlantic Blue™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths404 nm / 458 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP01868
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

The MOPC-21 immunoglobulin is useful as an isotype-matched control. The MOPC-21 immunoglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG1 antibodies. This antibody has been quality-tested for flow cytometry as negative control.

实验方案

Product Specific Protocols
FC protocol for Atlantic Blue™ IgG1 Isotype Control antibody AB-65124Download protocol
Standard Protocols
Click here to view our Standard Protocols